用琥珀酸二異辛酯磺酸鈉/ 環(huán)己烷反膠束體系合成憎水納米銀- 金復(fù)合顆粒,并用此納米銀- 金顆粒與聚乙烯醇縮丁醛構(gòu)成復(fù)合固酶膜基質(zhì),用溶膠- 凝膠法固定葡萄糖氧化酶,構(gòu)建葡萄糖生物傳感器. 實(shí)驗(yàn)表明,納米憎水銀- 金顆粒可以大幅度提高固定化酶的催化活性,響應(yīng)電流從相應(yīng)濃度的幾十納安增強(qiáng)到幾萬納安. 探討了納米顆粒效應(yīng)在固定化酶中所起的作用,為納米顆粒在生物傳感器領(lǐng)域中的應(yīng)用提供了可供參考的實(shí)驗(yàn)和理論依據(jù).
關(guān)鍵詞　納米憎水銀- 金顆粒,固定化酶,葡萄糖氧化酶,生物傳感器
Abstract 　Hydrophobic Ag2Au nanoparticles are produced in AOT [Aeroslo OT , sodium bis (22ethyhexyl ) sulfosuccinate ]2cyclohexane reverse micellar system. Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix , which is composed of hydrophobic Ag2Au particles and polyvinyl butyral ( PVB) by a sol2gel method. The experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilized enzyme. The current response is increased from tens of nanoamperometer (nA) to ten thousands of nanoamperometer to the same glucose concentration. The experiment and theory proved the function of nanoparticles in immobilization of enzyme. The catalysis of enzyme improved by nanoparticles was discussed. This research provides experimental and theoretical basis for preparation of useful glucose biosensor.
Keywords 　hydrophobic Ag2Au nanoparticles , immobilized enzyme , glucose oxidase , biosensor